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Understanding the TPHA Test

The TPHA (Treponema Pallidum Hemagglutination Assay) test is a blood-based diagnostic tool used to detect antibodies against Treponema pallidum, the bacterium responsible for syphilis. As a treponemal test, it does not detect the bacteria directly but identifies the immune system’s response, making it highly specific for confirming syphilis infection. The TPHA test is often performed after an initial screening test, such as the VDRL or RPR, to verify results. Once positive, the test usually remains so for life, even after successful treatment, as antibodies persist in the bloodstream. It plays an important role in diagnosis, guiding further evaluation and treatment decisions.

TPHA Test Measures

The TPHA test measures the presence of antibodies produced by the immune system in response to Treponema pallidum infection. It works on the principle of hemagglutination, where red blood cells coated with treponemal antigens clump together if antibodies are present in the patient’s blood sample. A positive result indicates exposure to syphilis at some point, while a negative result suggests no detectable antibodies. Since the test detects antibodies rather than the bacteria itself, it cannot distinguish between a current or past infection. For accurate diagnosis, the TPHA test is often interpreted alongside other tests such as VDRL or RPR.

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What is a TPHA Test, and Why is it Important?

The TPHA test, or Treponema Pallidum Hemagglutination Assay, is a blood-based diagnostic test used to detect antibodies against Treponema pallidum, the bacterium responsible for syphilis. It is a highly specific treponemal test, often performed to confirm results from screening tests like VDRL or RPR.

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Frequently Asked Questions

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What does a positive TPHA result mean?

Is a VDRL test the same as a TPHA test?

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How much does a TPHA test cost with MaxAtHome?

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Last Updated :
September 16, 2025

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